Build DNA "Let us make it together"
Picture
Your desire to help in improving health and quality life span by altering genes and interaction of genes using gene therapy, genome editing and novel nanovector/viral vector based gene delivery techniques interests me.

I execute projects related to tagging proteins by fluorescent tags, viral vector assembly, construction of library for TALENs, CRISPR/Cas designing and liposomal nanoparticle mediated gene delivery. I have expertise in designing single/multi-fragment clonings and DNA sequencing. My aim is to help in building DNA and thus, help in achieving broad targets of gene therapy and synthetic biology.

Skills and Techniques:
  • Designing and executing gene therapy and delivery projects (in vitro/in vivo)
  • Development of gene-based diagnostic methods
  • Design for targeting by genome editing nucleases - CRISPR/CAS system, TALENs (Transcription Activator Like Effector Nucleases) - CRISPR/Cas9 mediated gene deletion, base editing (A/T->G/C & C/G->T/A), gene activation/repression and homologous recombination experiments for disease correction.
  • Design of barcoded DNA molecules and high-throughput experiment design for assessing gene delivery.
  • Cre recombination system.
  • Droplet Digital PCR, real-time PCR
  • Gibson Assembly, multi-fragment and single fragment clonings
  • Designing primers for gene cloning, Sanger sequencing, deep sequencing, targeted genomic DNA amplification (humans, mouse etc.), qPCR, droplet digital PCR etc.
  • Tagging of genes by fluorescent proteins (EGFP, TdTomato, pmCherry etc.) for functional analysis of genes using IRES or 2A-peptides
  • Design of triplet primed PCR for screening of Myotonic Dystrophy I (DM1) disease
  • Design of southern blotting for DMPK gene, in Myotonic Dystrophy I Design of triplet primed PCR for screening of Myotonic Dystrophy I (DM1) disease
  • ​Genotyping experiments