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Build DNA "Let us make it together"
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Your desire to help in improving health and quality life span by altering genes and interaction of genes using gene therapy, genome editing and novel nanovector/viral vector based gene delivery techniques interests me.

I execute projects related to tagging proteins by fluorescent tags, viral vector assembly, construction of library for TALENs, CRISPR/Cas designing and liposomal nanoparticle mediated gene delivery. I have expertise in designing single/multi-fragment clonings and DNA sequencing. My aim is to help in building DNA and thus, help in achieving broad targets of gene therapy and synthetic biology.

Skills and Techniques:
  • Designing and executing gene therapy and delivery projects (in vitro/in vivo)
  • Development of gene-based diagnostic methods
  • Design for targeting by genome editing nucleases - CRISPR/CAS system, TALENs (Transcription Activator Like Effector Nucleases) - CRISPR/Cas9 mediated gene deletion, base editing (A/T->G/C & C/G->T/A), gene activation/repression and homologous recombination experiments for disease correction.
  • Design of barcoded DNA molecules and high-throughput experiment design for assessing gene delivery.
  • Cre recombination system.
  • Droplet Digital PCR, real-time PCR
  • Gibson Assembly, multi-fragment and single fragment clonings
  • Designing primers for gene cloning, Sanger sequencing, deep sequencing, targeted genomic DNA amplification (humans, mouse etc.), qPCR, droplet digital PCR etc.
  • Tagging of genes by fluorescent proteins (EGFP, TdTomato, pmCherry etc.) for functional analysis of genes using IRES or 2A-peptides
  • Design of triplet primed PCR for screening of Myotonic Dystrophy I (DM1) disease
  • Design of southern blotting for DMPK gene, in Myotonic Dystrophy I Design of triplet primed PCR for screening of Myotonic Dystrophy I (DM1) disease
  • ​Genotyping experiments

Publication list

"Free University of Brussels (VUB) - Research output of Kshitiz Singh"

"My Bibliography" from NCBI

"My Bibliography" from Google Scholar

Kshitiz Singh on ResearchGate

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